multocida directly from clinical swab specimens should be feasible. The results show that PCR detection of toxigenic P. multocida was recovered efficiently from inoculated swabs without inhibition of the PCR. In addition to accuracy, as required for a rapid direct specimen assay, toxigenic P. Results of an enzyme-linked immunosorbent assay for ToxA agreed with the other assays except for a negative reaction in one of the 19 isolates that the other assays identified as toxigenic. There was concordance of PCR results with (i) detection of toxA gene with colony blot hybridization, (ii) detection of ToxA protein with colony immunoblot analysis, and (iii) lethal toxicity of sonicate in mice in a test set of 40 swine diagnostic isolates. multocida and can detect fewer than 100 bacteria. The PCR amplification protocol is specific for toxigenic P. A PCR protocol which results in amplification of an 846-nucleotide segment of the toxA gene was developed. Like other types of viruses, bacteriophages vary a lot in their shape and genetic material. Bacteriophage, or phage for short, is a virus that infects bacteria. The feasibility of using PCR for accurate, rapid detection of toxigenic P. A tale of tails: Sialidase is key to success in a model of phage therapy against K1-capsulated Escherichia coli Bull, J. Lab 6: Bacteriophage Determining the Presence of a Pathogen Using Serial Dilutions, Linear Dilutions, and PCR. multocida isolates cannot be differentiated by morphology or standard biochemical reactions. A more rapid, accurate method to detect toxigenic Pasteurella multocida is needed for improved clinical diagnosis, farm biosecurity, and epidemiological studies.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |